Protein_Domain

Part:BBa_K5466030:Design

Designed by: Adrián Gómez Lara, Daniel Bulnes Roldán   Group: iGEM24_UMA-MALAGA   (2024-09-24)


Split mCerulean C-terminal. Saccharomyces cerevisiae codon optimized


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 84
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A flexible linker of that particular length has been added to provide enough freedom of movement for dimerization without being so long that it allows excessive movement, which could lead to fortuitous reconstitution.


Source

Secuence from https://www.fpbase.org/protein/mcerulean/